产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | OM641708 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Histone H3(mono methyl K36) [SR04-20]
Antibody Type
Primary Antibodies
Product
description
In eukaryotes,
DNA is wrapped around histone octamers to form the basic unit of chromatin
structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it
associates with approximately 200 base pairs of DNA to form the nucleosome.
The association of DNA with histones results in dense packing of chromatin,
which restricts proteins involved in gene transcription from binding to DNA.
Histone H3, the core protein of the nucleosome, becomes phosphorylated at the
end of prophase. The two major sites of phosphorylation are the
mitosis-specific site Ser10, and Ser28, both of which are extensively
phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone
H3. The nucleosome structure of histone H3 promotes N-terminal
phosphorylation in vitro.
More
Immunogen
recombinant
protein
Key Feature
Clonality
Monoclonal
Isotype
IgG
Host Species
Recombinant rabbit
Tested
Applications
WBICC/IF
WB:1:1,000-1:2,000
Species
Reactivity
HumanMouse
Concentration
1 mg/mL.
Target Information
Alternative
Names
Molecular
Weight(MW)
15 kDa
Cellular
Localization
Nucleus, Chromosome.
Database Links
SwissProt ID
P68431
Application
ICC:1:50-1:200
P84243
Q16695
Q6NXT2
Q71DI3
P84244
·
Application
Fig1: Western blot analysis of Histone H3(mono methyl K36) on different lysates using anti-Histone H3(mono methyl K36) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3
·
Application
Fig2: ICC staining Histone H3(mono methyl K36) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control |
NIH/3T3, MCF-7, Hela. |
Application Notes |
WB:1:1,000-1:2,000 |
Additional Information
Form |
Liquid |
Storage Instructions |
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer |
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |