产品展厅
Histone H3(mono methyl K36) [SR04-20]
  • 品牌:OminimAbs
  • 产地:上海
  • 型号:毫升
  • 货号:OM641708
  • 发布日期: 2024-09-14
  • 更新日期: 2024-10-09
产品详请
产地 上海
品牌 OminimAbs
保存条件
货号 OM641708
用途 实验研究
应用范围 科研实验
抗原来源 电询
保质期 2年
抗体名 电询
是否单克隆
克隆性
靶点 电询
适应物种 电询
形态 电询
宿主 电询
标记物 电询
包装规格 毫升
亚型 电询
标识物 电询
浓度 电询%
免疫原 电询
是否进口

Product Profile

Product Name

Histone H3(mono methyl K36) [SR04-20]

Antibody Type

Primary Antibodies

Product description

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser10, and Ser28, both of which are extensively phosphorylated in DNA-bound forms of histone H3 and in nucleosomal histone H3. The nucleosome structure of histone H3 promotes N-terminal phosphorylation in vitro.

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Immunogen

recombinant protein

Key Feature

Clonality

Monoclonal

Isotype

IgG

Host Species

Recombinant rabbit

Tested Applications

WBICC/IF

WB:1:1,000-1:2,000
ICC:1:50-1:200

Species Reactivity

HumanMouse

Concentration

1 mg/mL.

Target Information

Alternative Names

Molecular Weight(MW)

15 kDa

Cellular Localization

Nucleus, Chromosome.

Database Links

SwissProt ID

P68431
P84243
Q16695
Q6NXT2
Q71DI3
P84244

Application

·        

Application

Fig1: Western blot analysis of Histone H3(mono methyl K36) on different lysates using anti-Histone H3(mono methyl K36) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3

·        

Application

Fig2: ICC staining Histone H3(mono methyl K36) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Positive Control

NIH/3T3, MCF-7, Hela.

Application Notes

WB:1:1,000-1:2,000
ICC:1:50-1:200

Additional Information

Form

Liquid

Storage Instructions

Store at +4 after thawing. Aliquot store at -20 or -80. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.


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