|
- 品牌：OminimAbs
- 产地：上海
- 型号：毫升
- 货号：OM641708
- 发布日期： 2024-09-14
- 更新日期： 2024-10-09
| 产地 | 上海 |
| 品牌 | OminimAbs |
| 保存条件 | |
| 货号 | OM641708 |
| 用途 | 实验研究 |
| 应用范围 | 科研实验 |
| 抗原来源 | 电询 |
| 保质期 | 2年 |
| 抗体名 | 电询 |
| 是否单克隆 | 否 |
| 克隆性 | 否 |
| 靶点 | 电询 |
| 适应物种 | 电询 |
| 形态 | 电询 |
| 宿主 | 电询 |
| 标记物 | 电询 |
| 包装规格 | 毫升 |
| 亚型 | 电询 |
| 标识物 | 电询 |
| 浓度 | 电询% |
| 免疫原 | 电询 |
| 是否进口 | 否 |
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
· 
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
CREB1
CREB
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
·
Application
Western blot
analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B)
whole cell lysates.
·
Application
Immunofluorescent
analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were
permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with
3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the
primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a
hidified chamber. Cells were washed with PBST and incubated with a DyLight
594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical
analysis of CREB (pS142) staining in human breast cancer formalin fixed
paraffin embedded tissue section. The section was pre-treated using heat
mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was
then incubated with the antibody at room temperature and detected using an HRP
conjugated compact polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with DPX.
Application
Notes
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Additional Information
Form
Liquid
Storage
Instruction
Shipped at 4 C. Upon delivery aliquot and
store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate,
0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Profile
Product Name
CREB (phospho Ser142) antibody
Antibody Type
Primary Antibodies
Product
descriptio
Rabbit
Polyclonal antibody to CREB (phospho Ser142)This gene encodes a transcription
factor that is a member of the leucine zipper family of DNA binding proteins.
This protein binds as a homodimer to the cAMP-responsive element, an
octameric palindrome. The protein is phosphorylated by several protein
kinases, and induces transcription of genes in response to hormonal
stimulation of the cAMP pathway. Alternate splicing of this gene results in
two transcript variants encoding different isoforms. [provided by RefSeq, Jul
2008]
Hide
Immunogen
KLH-conjugated
synthetic peptide encompassing a sequence within the center region of human
CREB. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ICC/IFIHC-PWB
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200.
ICC/IF: 1:100 - 1:500:
Species
Reactivity
HumanRat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
cAMP responsive element binding protein 1
Target
CREB
Tissue
Specificity
Recognizes endogenous levels of CREB
(pS142) protein.
Application
CREB1
CREB
·
Application
Western blot analysis of CREB (pS142) expression in HeLa UV-treated (A); rat muscle (B) whole cell lysates.
·
Application
Immunofluorescent analysis of CREB (pS142) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 i??i??C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
·
Application
Immunohistochemical analysis of CREB (pS142) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
|
Application Notes |
WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. ICC/IF: 1:100 - 1:500: |
Additional Information
|
Form |
Liquid |
|
Storage Instruction |
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
|
Storage Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
-
中秋迎好礼 中秋迎好礼 随着国产3A大作《黑神话：悟空》的面世，现我司推出活动。 从即日起至2024年9月18日，购买ELISA试剂盒满五盒，即可申领《黑神话：悟空》数字标准版兑换码。 ... VIEW DETAILS
-
化学实验室守则小分享 一、实验前 在进行化学实验前，学生必须完成预习工作，了解实验的目的、步骤和注意事项。进入实验室前，应穿着适当的防护装备，如实验服、护目镜、实验手套等。此外，学生应提... VIEW DETAILS
-
犬肾素(REN)ELISA试剂盒在宠物健康监测中的重要作用 随着宠物健康管理的日益受重视，犬肾素(REN)ELISA试剂盒在宠物健康监测中发挥着不可或缺的作用。 犬肾素作为一种关键的激素，其水平的变化能够反映犬只肾脏的功能状态。而犬肾素(REN)ELISA试... VIEW DETAILS
-
上海笃玛生物科技有限公司2024年春节放假通知 尊敬的新老客户: 您好! 2024年春节即将到来，上海笃玛生物科技有限公司全体员工提前恭祝广大新老客户，新春快乐、生意兴隆、万事如意!非常感谢您长期以来对我们工作的理解... VIEW DETAILS
-
科研基础小知识分享，细胞传代 一、什么是细胞传代？ 细胞传代是细胞培养过程中的一项基本技术，是指将培养的细胞在一定的条件下进行分离、再培养的过程。传代过程中，细胞会逐渐生长，并填满整个培养容器，因此需要进... VIEW DETAILS
-
ELISA样品制备&收集指南 一、引言 酶联免疫吸附试验（ELISA）是一种广泛应用于生物学和医学研究的免疫分析方法。该方法通过将抗原或抗体固相化在载体表面，实现对目标分子的捕获和检测。在进行ELISA实验之前，... VIEW DETAILS
- 手机：15214367449
- Q Q：
