Product Profile

Product Name	Anti-CBFA2T3 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	CBFA2T3
Gene Synonyms	ETO2 MTG16 MTGR2 ZMYND4 RUNX1T3 Hide
Gene Full Name	CBFA2/RUNX1 partner transcriptional co-repressor 3
Gene Summary	This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010] Hide
Molecular Weight(MW)	71KD,63KD
Cellular Localizatio	Golgi apparatus, Membrane, Nucleus

Application

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WB

Western blot analysis using CBFA2T3 antibody against A431(1), NIH3T3 (2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CBFA2T3 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A549 cells using CBFA2T3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.