Product Profile

Product Name	Anti-PODXL antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	PODXL
Gene Synonyms	PC PDX PCLP Gp200 gp135 PCLP-1 PODXL1 Hide
Gene Full Name	podocalyxin like
Gene Summary	This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	59KD
Cellular Localizatio	Cell membrane, Cell projection, Membrane

Application

·        

WB

Western blot analysis using PODXL antibody against Hek293(1), Hepg2(2),MCF-7(3) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using PODXL antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of NIH3T3 cells using PODXL antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.