Product Profile

Product Name	Anti-RAG2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAIHCWB
	WB:1:200-1:2000 IHC:1:200-1:2000
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Protein A

Target Information

Gene Symbol	RAG2
Gene Synonyms	RAG-2
Gene Full Name	recombination activating 2
Gene Summary	This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	59KD
Cellular Localizatio	Nucleus

Application

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WB

Western blot analysis using RAG2 antibody against NIH3T3(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAG2 antibody with DAB staining.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:2000

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.