Product Profile

Product Name	Anti-SPARC antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SPARC
Gene Synonyms	ON ONT OI17 BM-40 Hide
Gene Full Name	secreted protein acidic and cysteine rich
Gene Summary	This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. Hide
Molecular Weight(MW)	35 kDa
Cellular Localizatio	Basement membrane, Extracellular matrix, Secreted

Application

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WB

Western blot analysis using SPARC antibody against K562(1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded renal carcinoma using SPARC antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using SPARC antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.