Product Profile

Product Name	Anti-MAP3K12 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MAP3K12
Gene Synonyms	DLK MUK ZPK ZPKP1 MEKK12 HP09298 Hide
Gene Full Name	mitogen-activated protein kinase kinase kinase 12
Gene Summary	This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described. Hide
Molecular Weight(MW)	93 kDa
Cellular Localizatio	Cell membrane, Cytoplasm, Membrane

Application

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WB

Western blot analysis using MAP3K12 antibody against MCF-7(1), PANC-1(2) cell lysate.

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ICC/IF

Immunofluorescence analysis of Hela cells using MAP3K12 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.