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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | OM641708 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Anti-RNF20 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
RNF20
Gene Synonyms
BRE1
Gene Full Name
calponin 1
Gene Summary
The protein encoded by this gene shares
similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene
is an E3 ubiquitin ligase that regulates chromosome structure by
monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor
and positively regulates the p53 tumor suppressor as well as numerous histone
H2A and H2B genes. In contrast, this protein also suppresses the expression
of several protooncogenes and growth-related genes, including many genes that
are induced by epidermal growth factor. This gene selectively suppresses the
expression of some genes by interfering with chromatin recruitment of
transcription elongation factor SII (TFIIS).
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Molecular
Weight(MW)
114 kDa
Cellular
Localizatio
Nucleus
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
BRE1A
hBRE1
·
WB
Western blot analysis using RNF20 antibody against MCF-7(1) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using RNF20 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of A549 cells using RNF20 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |