Product Profile

Product Name	Anti-NFKB antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Protein

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	NFKB1
Gene Synonyms	KBF1 EBP-1 NF-kB CVID12 NF-kB1 NFKB-p50 NFkappaB NF-kappaB NFKB-p105 NF-kappa-B1 NF-kappabeta Hide
Gene Full Name	nuclear factor kappa B subunit 1
Gene Summary	This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Aug 2020] Hide
Molecular Weight(MW)	50 kDa
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using NFKB antibody against Hela(1), A431(2),MCF-7(3),COS7(4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NFKB antibody with DAB staining.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.