Product Profile

Product Name	Anti-GARS antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	GARS1
Gene Synonyms	GARS HMN5 CMT2D DSMAV GlyRS HMN5A SMAD1 SMAJI Hide
Gene Full Name	glycyl-tRNA synthetase 1
Gene Summary	This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] Hide
Molecular Weight(MW)	83 kDa
Cellular Localizatio	Cell projection, Cytoplasm, Mitochondrion, Secreted

Application

·

WB

Western blot analysis using GARS antibody against Hela(1), Jurkat(2) cell lysate.

·

IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using GARS antibody with DAB staining.

·

ICC/IF

Immunofluorescence analysis of Hela cells using GARS antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.

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