Product Profile

Product Name	Anti-SDHA antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFWB
	WB:1:200-1:2000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SDHA
Gene Synonyms	FP PGL5 SDH1 SDH2 SDHF CMD1GG MC2DN1 NDAXOA Hide
Gene Full Name	succinate dehydrogenase complex flavoprotein subunit A
Gene Summary	This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] Hide
Molecular Weight(MW)	73 kDa
Cellular Localizatio	Membrane, Mitochondrion, Mitochondrion inner membrane

Application

·        

WB

Western blot analysis using SDHA antibody against Hela(1), NIH/3T3(2), Jurkat(3) cell lysate.

·        

ICC/IF

Immunofluorescence analysis of A549 cells using SDHA antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.