Product Profile

Product Name	Anti-MEK1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MAP2K1
Gene Synonyms	MEL CFC3 MEK1 MKK1 MAPKK1 PRKMK1 Hide
Gene Full Name	mitogen-activated protein kinase kinase 1
Gene Summary	The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	43 kDa
Cellular Localizatio	Cytoplasm, Cytoskeleton, Membrane, Nucleus

Application

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WB

Western blot analysis using MEK1 antibody against HepG2(1),PANC-1(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MEK1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using MEK1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.