Product Profile

Product Name	Anti-Aurora A antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AURKA
Gene Synonyms	AIK ARK1 AURA BTAK STK6 STK7 STK15 PPP1R47 More
Gene Full Name	aurora kinase A
Gene Summary	The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] More
Molecular Weight(MW)	46 kDa
Cellular Localizatio	Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

Application

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WB

Western blot analysis using Aurora A antibody against MCF-7(1), C2C12(2) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Aurora A antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using Aurora A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.