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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | OM641708 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Anti-Cdk4 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
CDK4
Gene Synonyms
CMM3
Gene Full Name
cyclin dependent kinase 4
Gene Summary
The protein encoded by this gene is a
member of the Ser/Thr protein kinase family. This protein is highly similar
to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a
catalytic subunit of the protein kinase complex that is important for cell
cycle G1 phase progression. The activity of this kinase is restricted to the
G1-S phase, which is controlled by the regulatory subunits D-type cyclins and
CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the
phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene
as well as in its related proteins including D-type cyclins, p16(INK4a) and
Rb were all found to be associated with tumorigenesis of a variety of
cancers. Multiple polyadenylation sites of this gene have been reported.
[provided by RefSeq, Jul 2008]
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Molecular
Weight(MW)
34 kDa
Cellular
Localizatio
Cytoplasm, Membrane, Nucleus
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
PSK-J3
·
WB
Western blot analysis using Cdk4 antibody against THP-1(1), K562(2) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using Cdk4 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of Hela cells using Cdk4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |