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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | 电询 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Anti-MEKK3 antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
MAP3K3
Gene Synonyms
MEKK3
Gene Full Name
mitogen-activated protein kinase kinase
kinase 3
Gene Summary
This gene product is a 626-amino acid
polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is
closely related to those of several other kinases, including mouse Mekk2,
tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb
transcript that appears to be ubiquitously expressed. This protein directly
regulates the stress-activated protein kinase (SAPK) and extracellular
signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2
respectively; it does not regulate the p38 pathway. In cotransfection assays,
it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent
reporter gene, consistent with a role in the SAPK pathway. Alternatively
spliced transcript variants encoding distinct isoforms have been observed.
[provided by RefSeq, Jul 2008]
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Molecular
Weight(MW)
71 kDa
Cellular
Localizatio
Cytosol, cytoplasm
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
MAPKKK3
·
WB
Western blot analysis using MEKK3 antibody against K562(1),Thp-1(2) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MEKK3 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of A549 cells using MEKK3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |