Product Profile

Product Name	Anti-ERK2 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	MAPK1
Gene Synonyms	ERK p38 p40 p41 ERK2 ERT1 NS13 ERK-2 MAPK2 PRKM1 PRKM2 P42MAPK p41mapk p42-MAPK Hide
Gene Full Name	mitogen-activated protein kinase 1
Gene Summary	This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] Hide
Molecular Weight(MW)	41 kDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Cytoskeleton, Membrane, Nucleus

Application

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WB

Western blot analysis using ERK2 antibody against HEK293(1), Jurkat(2), A549(3), A431(4) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ERK2 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of MG-63 cells using ERK2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.