Product Profile

Product Name	Anti-CHK2 antibody
Antibody Type	Primary Antibodies
Immunogen	Recombinent Proteins

Key Feature

Clonality	Monoclonal
Isotype	IgG
Host Species	Mouse
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanRabbit
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CHEK2
Gene Synonyms	CDS1 LFS2 RAD53 hCds1 HuCds1 PP1425 Hide
Gene Full Name	checkpoint kinase 2
Gene Summary	In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Hide
Molecular Weight(MW)	61 kDa
Source	Mouse
Cellular Localizatio	Nucleus

Application

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WB

Western blot analysis using CHK2 antibody against Hep G2 (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CHK2 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of HepG2 cells using CHK2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.