Product Profile

Product Name	Anti-NM23 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	NME1
Gene Synonyms	NB AWD NBS GAAD NDKA NM23 NDPKA NDPK-A NM23-H1 More
Gene Full Name	NME/NM23 nucleoside diphosphate kinase 1
Gene Summary	This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] More
Molecular Weight(MW)	17 KDa
Source	Rabbit
Cellular Localizatio	Cytoplasm, Nucleus

Application

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WB

Western blot analysis using NM23 antibody against Jurkat (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded renal carcinomar tissues using NM23 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of HepG2 cells using NM23 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.