Product Profile

Product Name	Anti-CK5 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	KRT5
Gene Synonyms	K5 CK5 DDD DDD1 EBS2 KRT5A Hide
Gene Full Name	keratin 5
Gene Summary	The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	62 KDa
Source	Rabbit
Cellular Localizatio	Cytoskeleton，Cytosol，Nucleus，Plasma Membrane，Extracellular region or secreted

Application

        

WB

Western blot analysis using CK5 antibody against A431 (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CK5 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of Hela cells using CK5 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.