Product Profile

Product Name	Anti-Iba1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	AIF1
Gene Synonyms	IBA1 IRT1 AIF-1 IRT-1 More
Gene Full Name	allograft inflammatory factor 1
Gene Summary	This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] More
Molecular Weight(MW)	17 kDa（Observed:55 kDa）
Source	Rabbit
Cellular Localizatio	Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Application

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WB

Western blot analysis using Iba1 antibody against Raji (1), A431 (2), HepG2 (3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using Iba1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of PANC-1 cells using Iba1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.