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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | OM641708 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name |
Anti-E2F1 antibody |
Antibody Type |
Primary Antibodies |
Immunogen |
Polypeptide |
Key Feature
Clonality |
Polyclonal |
Isotype |
IgG |
Host Species |
Rabbit |
Tested Application |
ELISAICC/IFIHCWB |
WB:1:200-1:2000 |
|
Species Reactivity |
HumanMouseRat |
Concentration |
1 mg/ml |
Purification |
Protein A |
Target Information
Gene Symbol |
E2F1 |
Gene Synonyms |
RBP3 Hide |
Gene Full Name |
E2F transcription factor 1 |
Gene Summary |
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008] Hide |
Molecular Weight(MW) |
47 KDa |
Source |
Rabbit |
Cellular Localizatio |
Nucleus |
Application
·
WB
Western blot analysis using E2F1 antibody against A549 (1), CaCo-2 (2) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using E2F1 antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of Hela cells using E2F1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |