Product Profile

Product Name	Anti-CYP11A1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	CYP11A1
Gene Synonyms	CYP11A CYPXIA1 P450SCC
Gene Full Name	cytochrome P450 family 11 subfamily A member 1
Gene Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] Hide
Molecular Weight(MW)	60 kDa
Source	Rabbit
Cellular Localizatio	Membrane, Mitochondrion, Mitochondrion inner membrane

Application

·        

WB

Western blot analysis using CYP11A1 antibody against Hep G2 (1), Jurkat (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissues using CYP11A1 antibody with DAB staining.

·        

ICC/IF

Immunofluorescence analysis of HepG2 cells using CYP11A1 antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.