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产地 | 上海 |
品牌 | OminimAbs |
保存条件 | |
货号 | OM641708 |
用途 | 实验研究 |
应用范围 | 科研实验 |
抗原来源 | 电询 |
保质期 | 2年 |
抗体名 | 电询 |
是否单克隆 | 否 |
克隆性 | 否 |
靶点 | 电询 |
适应物种 | 电询 |
形态 | 电询 |
宿主 | 电询 |
标记物 | 电询 |
包装规格 | 毫升 |
亚型 | 电询 |
标识物 | 电询 |
浓度 | 电询% |
免疫原 | 电询 |
是否进口 | 否 |
Product Profile
Product Name
Anti-GAPDH antibody
Antibody Type
Primary Antibodies
Immunogen
Polypeptide
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested
Application
ELISAICC/IFIHCWB
WB:1:200-1:2000
Species
Reactivity
HumanMouseRat
Concentration
1 mg/ml
Purification
Protein A
Target Information
Gene Symbol
GAPDH
Gene Synonyms
G3PD
Gene Full Name
glyceraldehyde-3-phosphate dehydrogenase
Gene Summary
This gene encodes a member of the
glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein
has been identified as a moonlighting protein based on its ability to perform
mechanistically distinct functions. The product of this gene catalyzes an
important energy-yielding step in carbohydrate metabolism, the reversible
oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of
inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded
protein has additionally been identified to have uracil DNA glycosylase
activity in the nucleus. Also, this protein contains a peptide that has
antimicrobial activity against E. coli, P. aeruginosa, and C. albicans.
Studies of a similar protein in mouse have assigned a variety of additional
functions including nitrosylation of nuclear proteins, the regulation of mRNA
stability, and acting as a transferrin receptor on the cell surface of
macrophage. Many pseudogenes similar to this locus are present in the human
genome. Alternative splicing results in multiple transcript variants.
[provided by RefSeq, Nov 2014]
Hide
Molecular
Weight(MW)
36 kDa
Source
Rabbit
Cellular
Localizatio
Cytoplasm, Cytoskeleton, Membrane,
Nucleus
Application
IHC:1:200-1:1000
ICC/IF:1:100-1:500
GAPD
HEL-S-162eP
·
WB
Western blot analysis using GAPDH antibody against COS-7 (1), C2C12 (2) cell lysate.
·
IHC
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using GAPDH antibody with DAB staining.
·
ICC/IF
Immunofluorescence analysis of Hela cells using GAPDH antibody(green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Application Notes |
WB:1:200-1:2000 |
Additional Information
Form |
Liquid |
Storage Instruction |
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
Storage Buffer |
Purified antibody in PBS with 0.05% sodium azide. |