Product Profile

Product Name	Anti-SREBP1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAIHCWB
	IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SREBF1
Gene Synonyms	HMD IFAP2 SREBP1 bHLHd1 Hide
Gene Full Name	sterol regulatory element binding transcription factor 1
Gene Summary	This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] Hide
Molecular Weight(MW)	122 kDa
Source	Rabbit
Cellular Localizatio	Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane.

Application

·        

WB

Western blot analysis using SREBP1 antibody against NIH3T3 (1), Jurkat (2) cell lysate.

·        

IHC

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using SREBP1 antibody with DAB staining.

Positive Control	WB::1:200-1:2000||IHC::1:200-1:1000
Application Notes	IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.