Product Profile

Product Name	Anti-GRP78 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	HSPA5
Gene Synonyms	BIP HSPA5 HEL-S-89n
Gene Full Name	heat shock protein family A (Hsp70) member 5
Gene Summary	The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020] Hide
Molecular Weight(MW)	78 kDa（Observed:54 kDa)
Source	Rabbit
Cellular Localizatio	Cytoplasm; Endoplasmic reticulum

Application

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WB

Western blot analysis using GRP78 antibody against Hela (1), NIH 3T3 (2), Hep G2 (3) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded cervica cancer tissues using GRP78 antibody with DAB staining