Product Profile

Product Name	Anti-ZO 1 antibody
Antibody Type	Primary Antibodies
Immunogen	Polypeptide

Key Feature

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Application	ELISAICC/IFIHCWB
	WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500
Species Reactivity	HumanMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	TJP1
Gene Synonyms	Tight junction protein 1 Tight junction protein ZO 1 TJP1 ZO 1 ZO1 ZO-1 Zona occludens protein 1 Zonula occludens protein 1 Hide
Gene Full Name	tight junction protein 1
Gene Summary	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] Hide
Molecular Weight	195 kDa
Source	Rabbit
Cellular Localizatio	Cell junction; Cell membrane; Cell projection; Gap junction; Membrane; Tight junction

Application

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WB

Western blot analysis using ZO 1 antibody against A431 (1) cell lysate.

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IHC

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ZO 1 antibody with DAB staining.

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ICC/IF

Immunofluorescence analysis of A431 cells using ZO 1 (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes

WB:1:200-1:2000 IHC:1:200-1:1000 ICC/IF:1:100-1:500

Additional Information

Form	Liquid
Storage Instruction	Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer	Purified antibody in PBS with 0.05% sodium azide.