LB Broth with agar (Luria low salt)

别名:Luria Agar Base (Miller’s Modification)

属性
等级
for molecular biology

质量水平
200

无菌性
non-sterile

形式
powder

组成
Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)
microbiological culture: suitable

pH值（酸碱度）
6.8-7.2(3.05% solution)

application(s)
food and beverages
microbiology

储存温度
room temp

适用性
nonselective for Escherichia coli
nonselective for coliforms

说明
一般描述
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
应用
LB Broth with agar (Luria low salt) has been used:
for culturing E. coli DH10B cells. [1]
in the isolation of diazotrophic bacteria. [2]
for transformation of competent E. coli strain Stbl3 for cloning. [3]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
特点和优势
Luria′s low salt LB powder with agar provides:
Easy scale-up using larger package sizes
A budget-friendly alternative to pre-poured plates
Standard formulation
制备说明
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

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SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元