推荐：KOVACS′ indole reagent

别名:4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles

属性
质量水平
200

形式
liquid

包装
pkg of 100 mL

储存条件
(No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition)

technique(s)
microbe id | specific enzyme detection: suitable

颜色
yellow

pH值（酸碱度）
<1 (20 °C in H2O)

转变温度
flash point 36 °C

密度
0.92 g/cm3 at 20 °C

application(s)
microbiology

储存温度
2-8°C

适用性
Escherichia coli
enterovirulent E. coli (EEC) spp.

说明
一般描述
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
应用
Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.
分析说明
Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).
其他说明
The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元