推荐：革兰氏藏红 溶液 for microscopy

别名:藏红 T 溶液

Empirical Formula (Hill Notation):C20H19ClN4

CAS号:477-73-6

分子量:350.84

Beilstein:3924099

MDL编号:MFCD00011759

PubChem化学物质编号:329770368

NACRES:MA.02

属性
等级
for microscopy

质量水平
100

形式
liquid

保质期
limited shelf life, expiry date on the label

组成
ethanol, 10%

technique(s)
microbe id | staining: suitable

折射率
n20/D 1.339

密度
0.986 g/mL at 20 °C

εmax
4.0 at 532 nm in 50% ethanol

抗生素 谱
Gram-negative bacteria
Gram-positive bacteria

application(s)
diagnostic assay manufacturing
hematology
histology

储存温度
room temp

SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N

说明
一般描述
Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.[1]
应用
Gram’s safranin solution has been used in the following applications:
as a non-toxic replacement for crystal violet for the quantification of biofilm formation[2]
to develop a hyaluronic acid visualization method[3]
to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole[4]
特点和优势
Ready-to-use solution.
Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems.
原理
Gram staining enables the differentiation of bacteria into Gram-positive and Gram-negative. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with crystal violet, and then with iodine solution, which forms a dye-iodine complex. This complex is retained in the multi-layer mureine structure of the cell wall of Gram-positive bacteria after decolorization, imparting a blue-violet color. The cell wall of Gram-negative bacteria comprises a single-layered murein structure that does not retain the dye-iodine complex, allowing the uptake of safranine counterstain, and imparting a pink-red color.

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发表刊物        影响因子           奖励金额
SCI期刊杂志      1.0≤1F＜3.0          200元
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SCI期刊杂志      6.0≤1F＜10.0         600元
SCI期刊杂志      1F≥10                1000元