产地 | 上海 |
保存条件 | 2.8℃ |
品牌 | DM |
货号 | DM-P5449 |
用途 | 科研与实验 |
检测方法 | 吸附测定法 |
保质期 | 6个月 |
适应物种 | 猪 |
检测限 | 猪 |
数量 | 1000 |
包装规格 | 96T/48T |
标记物 | 电询 |
样本 | 血清,细胞,细胞上清液、体液等 |
应用 | 科研试剂 |
是否进口 | 否 |
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
适用的样本类型
血清、血浆、细胞上清液、胸腹水、脑脊液、处理后的固体样本等重复性:板内、板间变异系数均小于 15%
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被相关指标的抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并 洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的相关指标呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解 冻并确保样品均匀地充分解冻。
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
8. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
洗板方法
1.手工洗板:甩尽孔内液体,每孔加满洗涤液,静置 1min 后甩尽孔内液体,在吸水纸上拍干,如此洗板 5次。 人脂联素(ADP/Acrp30)ELISA试剂盒多种属供应
操作步骤
1.从室温平衡 20min 后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回 4℃。凡在本公司购买ELISA试剂盒,可提供免费待测服务,视样本数量而定出结果时间,一般一周内出检测结果!
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